The BMP/BMPR/Smad pathway directs expression of the erythroid-specific EKLF and GATA1 transcription … - Full text - MIT Libraries
CA Adelman, S Chattopadhyay, JJ Bieker - Development, 2002 - dev.biologists.org
... PCR primers for EKLF, HPRT, bh1, ßmaj, Bra, BMP4, GATA2 and GATA1 have been previously
described (Weiss et al., 1994; Johansson and Wiles, 1995; Southwood et ...
Cited by 11 - Web Search - dev.biologists.org - ncbi.nlm.nih.gov

Loss of gata1 but not gata2 converts erythropoiesis to myelopoiesis in zebrafish embryos - Full text - MIT Libraries
JL Galloway, RA Wingert, C Thisse, B Thisse, LI … - Dev Cell, 2005 - ncbi.nlm.nih.gov
... to this fate change, expression of many erythroid genes was found to be differentially
dependent on Gata1 alone, on both Gata1 and Gata2, or independent of ...
Cited by 2 - Web Search

Regulatory elements of the EKLF gene that direct erythroid cell-specific expression during mammalian … - Full text - MIT Libraries
L Xue, X Chen, Y Chang, JJ Bieker - Blood, 2004 - bloodjournal.org
... that GATA2 interacts with the EKLF promoter early in hematopoiesis, but that it
is replaced by GATA1 later on, analogous to a GATA2/GATA1 transition model ...
Cited by 3 - Web Search - bloodjournal.org - ncbi.nlm.nih.gov - ncbi.nlm.nih.gov

Molecular cloning and characterization of the GATA 1 cofactor human FOG 1 and assessment of its … - Full text - MIT Libraries
K Freson, C Thys, C Wittewrongel, J Vermylen, M … - Human Genetics, 2003 - springerlink.com
... Recently, it has been shown in mice that, during early megakaryopoiesis, Fog1 also
in- teracts with Gata2 when Gata1 is absent (Chang et al. 2002). ...
Cited by 2 - Web Search - ncbi.nlm.nih.gov - ncbi.nlm.nih.gov

protein GATAl - Full text - MIT Libraries
H OSADA, G GRUTZ, H AXELSON, A FORSTER, TH … - Proc. Natl. Acad. Sci. USA, 1995 - pnas.org
... a 1.24-kb fragment comprising the complete coding region of mouse GATA1, which was ...
extending from codon 230 to the C terminus of human GATA2 [ampli- fied by ...
Cited by 85 - Web Search - pnas.org - pubmedcentral.nih.gov - ncbi.nlm.nih.gov - all 5 versions »

GATA1 Function, a Paradigm for Transcription Factors in Hematopoiesis - Full text - MIT Libraries
R Ferreira, K Ohneda, M Yamamoto, S Philipsen - Mol Cell Biol, 2005 - mcb.asm.org
... The same report also shows that GATA2, together with CBP, can bind to the same regions
as GATA1 in its absence and that displacement of GATA2 by GATA1 is the ...
Cited by 1 - Web Search - pubmedcentral.nih.gov - ncbi.nlm.nih.gov

Immortalization of yolk sac-derived precursor cells - Full text - MIT Libraries
WM Yu, TS Hawley, RG Hawley, CK Qu - Blood, 2002 - bloodjournal.org
... factor, stem cell factor, basic fibroblast growth factor, and interleukin 6. They
also expressed the transcription factors SCL, GATA2, GATA1, PU.1, and c-myb. ...
Cited by 2 - Web Search - bloodjournal.org - bloodjournal.org - ncbi.nlm.nih.gov - all 6 versions »

In Silico Identification of Regulatory Elements of GRIN 1 Genes
MK Mejia-Guerra, LR Lareo - Omics A Journal of Integrative Biology, 2005 - dx.doi.org
... GATA2, GATA1 GATA3 600 to 500 GATA1 Sp1 Mkx-2 500 to 400 E47 c-Ets STATx E12
STATx ... 300 to 200 GATA1 GATA1, GATAX GATA1, GATAX GATA2 GATA2 ...
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Table 1. List of Transcription Factors of Which TFBSs Were Found in the Alk1 Regulatory Fragment and …
AP VEGFR, C VEGFR, G ICAM - ahavj.ahajournals.org
... NF- B, VEGFR2, 17 PECAM, 19 ICAM2 20, GATA, vWF 21, VEGFR2 22. GATA1, ICAM2 15,
GATA2, PECAM, 23 endothelin1, 24–26 ICAM2, 15 eNOS 16, ETS, ICAM2 15, Tie2, 27 VEGFR2 ...
Web Search

STAT6-Dependent Differentiation and Production of IL-5 and IL-13 in Murine NK2 Cells - Full text - MIT Libraries
T Katsumoto, M Kimura, M Yamashita, H Hosokawa, K … - The Journal of Immunology, 2004 - jimmunol.org
... with similar results. Expression of GATA3, GATA2, GATA1, ROG, granzyme
B, perforin 1, and IL-12R 2 in differentiated NK subsets. ...
Web Search - jimmunol.org - jimmunol.org - ncbi.nlm.nih.gov - all 5 versions »

Reciprocal interactions of Pit1 and GATA2 mediate signaling gradient-induced determination of … - Full text - MIT Libraries
JS Dasen, SM O’Connell, SE Flynn, M Treier, AS … - Cell, 1999 - rosenfeldlab.ucsd.edu
... structural analysis of GATA1 (Omichinski et al., ated a mutation in the homeodomain
of Pit1 (N51A, (D) Mapping of the Pit1 interaction interface with GATA2. ...
Cited by 79 - View as HTML - Web Search - molecular.biosciences.wsu.edu - pharmacology.cwru.edu - all 7 versions »

Gata2 and Gata3: novel markers for early embryonic polarity and for non-neural ectoderm in the chick … - Full text - MIT Libraries
G Sheng, CD Stern - Mech. Dev, 1999 - sternlab.anat.ucl.ac.uk
... Gata family of zinc ®nger transcription factors contains six members, Gata1±6. Gata ...
More recently, Gata2 and Gata3 have also been suggested to play a role in ...
Cited by 13 - View as HTML - Web Search - ingentaconnect.com - ncbi.nlm.nih.gov - all 4 versions »

Identification of novel vascular endothelial–specific genes by the microarray analysis of the … - Full text - MIT Libraries
S Sumanas, T Jorniak, S Lin - Blood, 2005 - bloodjournal.org
... 7-9 Transcription factors including gata2, gata1, cmyb, and transcription
factor–interacting proteins such as lmo2 gradually restrict the subpopulation of ...
Web Search - bloodjournal.org - ncbi.nlm.nih.gov

The Zebrafish moonshine Gene Encodes Transcriptional Intermediary Factor 1γ, an Essential Regulator … - Full text - MIT Libraries
DG Ransom, N Bahary, K Niss, D Traver, C Burns, NS … - PLoS Biol, 2004 - dx.doi.org
... arrow). Whole-mount in situ hybridization at 22 hpf including scl, gata2,
gata1, ikaros, and myb in mon tg234 mutants. Expression ...
Cited by 2 - Web Search - medicine.plosjournals.org - plbi.allenpress.com - pubmedcentral.nih.gov - all 8 versions »

Self-Association of Gata1 Enhances Transcriptional Activity In Vivo in Zebra Fish Embryos - Full text - MIT Libraries
K Nishikawa, M Kobayashi, A Masumi, SE Lyons, BM … - Mol. Cell. Biol, 2003 - mcb.asm.org
... in the zebra fish Gata1 protein, which are important for self-association, are
conserved among vertebrate hematopoietic Gata factors Gata1, Gata2, and Gata3. ...
Cited by 5 - Web Search - pubmedcentral.nih.gov - mcb.asm.org - ncbi.nlm.nih.gov - all 5 versions »

Abnormalities of the genitourinary tract in female mice lacking GATA5 - Full text - MIT Libraries
JD Molkentin, KM Tymitz, JA Richardson, EN Olson - Mol Cell Biol, 2000 - mcb.asm.org
... GATA1, GATA2, and GATA3 are expressed predominantly in hematopoietic cell
lineages. GATA1 is required for erythroid and megakaryocyte ...
Cited by 36 - Web Search - pubmedcentral.nih.gov - mcb.asm.org - ncbi.nlm.nih.gov

Functional Analysis of Human Hematopoietic Stem Cell Gene Expression Using Zebrafish - Full text - MIT Libraries
CE Eckfeldt, EM Mendenhall, CM Flynn, TF Wang, MA … - PLoS Biol, 2005 - medicine.plosjournals.org
... signaling) and commitment (vascular endothelial growth factor signaling; flk1, also
known as kdr; lmo2; scl, also known as tal1; gata2; gata1) are conserved ...
Cached - Web Search - plme.allenpress.com - biology.plosjournals.org - pubmedcentral.nih.gov

Differences in the chromatin structure and cis-element organization of the human and mouse GATA1 … - Full text - MIT Libraries
V Valverde-Garduno, B Guyot, E Anguita, I Hamlett, … - Blood, 2004 - bloodjournal.org
... These include GATA sites (both GATA1 and GATA2 have been implicated in promoting
GATA1 expression 16,49 ) and E-boxes that can bind the hematopoietic ...
Cited by 3 - Web Search - dx.doi.org - bloodjournal.org - ncbi.nlm.nih.gov

Hematopoietic regulatory domain of gata1 gene is positively regulated by GATA1 protein in zebrafish … - Full text - MIT Libraries
M Kobayashi, K Nishikawa, M Yamamoto - Development, 2001 - dev.biologists.org
... It is intriguing to note that not only the gata1 gene, but other family members
of the hematopoietic GATA factor family, gata2 and gata3, contain the first ...
Cited by 17 - Web Search - dev.biologists.org - ncbi.nlm.nih.gov - ncbi.nlm.nih.gov

TRANSCRIPTIONAL REGULATION OF T LYMPHOCYTE DEVELOPMENT AND FUNCTION - Full text - MIT Libraries
CT Kuo, JM Leiden - Annual Review of Immunology, 1999 - immunol.annualreviews.org
... GATA1 (28, 29), GATA2 (30, 31), and GATA3 (32, 33) belong to one subfamily and are
expressed in overlapping patterns in hematopoietic cells, whereas GATA4 (34 ...
Cited by 125 - Web Search - immunol.annualreviews.org - ncbi.nlm.nih.gov

Characterization of whole genome radiation hybrid mapping resources for non-mammalian vertebrates - Full text - MIT Libraries
C Kwok, RM Korn, ME Davis, DW Burt, R Critcher, L … - Nucleic Acids Research, 1998 - nar.oupjournals.org
... Zebrafish cDNAs for Tie1, GATA1, GATA2, TTG2, PU.1, c-myb, embryonic [alpha]-globin
(hbe1 on LG3), [alpha]x-globin (hbx on LG12) and retinoic acid receptor 2 ...
Cited by 62 - Web Search - pubmedcentral.nih.gov - ingentaconnect.com - ncbi.nlm.nih.gov - all 6 versions »

Gonadal differentiation, sex determination and normal Sry expression in mice require direct … - Full text - MIT Libraries
SG Tevosian, KH Albrecht, JD Crispino, Y Fujiwara, … - Development, 2002 - dev.biologists.org
... The GATA zinc-finger transcription factors (designated GATA1 to GATA6) recognize
the ... Gata2 (GATA-binding protein 2) is expressed between E10.5-15.5 in XX ...
Cited by 31 - Web Search - dev.biologists.org - ncbi.nlm.nih.gov

A tissue-specific knockout reveals that Gata 1 is not essential for Sertoli cell function in the … - Full text - MIT Libraries
F Lindeboom, N Gillemans, A Karis, M Jaegle, D … - Nucleic Acids Research, 2003 - nar.oupjournals.org
... Furthermore, negative regulation of Gata2 transcription by Gata1 has been
suggested (35). Finally, a transgene driving expression ...
Cited by 5 - Web Search - pubmedcentral.nih.gov - ingentaconnect.com - dspace.ubib.eur.nl - all 11 versions »

Phosphorylation of GATA2 by Akt Increases Adipose Tissue Differentiation and Reduces Adipose Tissue … - Full text - MIT Libraries
R Menghini, V Marchetti, M Cardellini, ML Hribal, … - Circulation, 2005 - circ.ahajournals.org
... properties. An Akt consensus motif was reported to be common to GATA1, GATA2,
and GATA3 and conserved among human, rat, and mouse. 20 ...
Web Search - circulationaha.org - circ.ahajournals.org - ncbi.nlm.nih.gov - all 5 versions »

Developmental stage− selective effect of somatically mutated leukemogenic transcription factor … - Full text - MIT Libraries
Z Li, FJ Godinho, JH Klusmann, M Garriga-Canut, C … - Nature Genetics, 2005 - nature.com
... Among the genes that are not properly downregulated in Gata1 N megakaryocytes (Fig.
3n), five transcription factors (Myc, Myb, GATA2, PU.1 and ZNFN1A1 (also ...
Cited by 2 - Web Search - nature.com - ncbi.nlm.nih.gov

Role of NFAT proteins in IL13 gene transcription in mast cells - Full text - MIT Libraries
S Monticelli, DC Solymar, A Rao - J. Biol. Chem, 2004 - jbc.org
... GATA expression vectors were obtained from S. Orkin (GATA1 and GATA2) and K. Murphy
(GATA3). ... GATA1 and GATA2 also have different functions in mast cells. ...
Cited by 2 - Web Search - dx.doi.org - ijmm.org - ncbi.nlm.nih.gov - all 5 versions »

Human Genome and Diseases: Review Transcription factor GATA3 and the human HDR syndrome - Full text - MIT Libraries
H Van Esch, K Devriend - Cell. Mol. Life Sci, 2001 - springerlink.com
... GATA3, GATA1 and GATA2 are necessary factors for haematopoiesis: gene disruption
of any of them results in major haematopoietic defects in the mouse [19, 20, 45 ...
Cited by 4 - Web Search - ncbi.nlm.nih.gov

Transcription factor expression in cell lines derived from natural killer-cell and natural killer- …
Y Matsuo, HG Drexler, A Harashima, A Okochi, N … - Hum Cell, 2004 - ncbi.nlm.nih.gov
... The present study analyzed the expression of the following 13 TFs: AML1, CEBPA,
E2A, ETS1, GATA1, GATA2, GATA3, IKAROS, IRF1, PAX5, PU1, TBET and TCF1 in 7 ...
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Selective impairment of platelet activation to collagen in the absence of GATA1 - Full text - MIT Libraries
SC Hughan, Y Senis, D Best, A Thomas, J Frampton, … - Blood, 2005 - bloodjournal.org
... 1 GATA1 is expressed in red cells, megakaryocytes, eosinophils, and mast cells where
it is required for terminal differentiation. 1 GATA2 is expressed in early ...
Web Search - bloodjournal.org - ncbi.nlm.nih.gov

The transcription factor GATA6 is essential for branching morphogenesis and epithelial cell … - Full text - MIT Libraries
R Keijzer, M van Tuyl, C Meijers, M Post, D … - Development, 2001 - dev.biologists.org
... First, GATA1, GATA2 and GATA3, which all have unique functions in the hematopoietic
system (Leonard et al., 1993; Pandolfi et al., 1995; Pevny et al., 1991 ...
Cited by 30 - Web Search - lungtranscriptome.bwh.harvard.edu - ncbi.nlm.nih.gov - dspace.ubib.eur.nl - all 9 versions »

GATA Motifs Regulate Early Hematopoietic Lineage-Specific Expression of the Gata2 Gene
M Kobayashi-Osaki, O Ohneda, N Suzuki, N Minegishi … - mcb.asm.org
... Gata2 gene expression was downregulated when GATA-1 was induced during erythroid
cell maturation (50), whereas expression was markedly induced when the Gata1 ...
Web Search - mcb.asm.org

Multiple, Distant Gata2 Enhancers Specify Temporally and Tissue-Specific Patterning in the … - Full text - MIT Libraries
M Khandekar, N Suzuki, J Lewton, M Yamamoto, JD … - Mol Cell Biol, 2004 - mcb.asm.org
... a cooperative delocalized enhancer, as appears to be required for Gata2 hematopoietic
activity ... erythroid enhancer activity in the first intron of Gata1 is only ...
Web Search - pubmedcentral.nih.gov - mcb.asm.org - ncbi.nlm.nih.gov

Non-cell autonomous requirement for the bloodless gene in primitive hematopoiesis of zebrafish - Full text - MIT Libraries
EC Liao, NS Trede, D Ransom, A Zapata, M Kieran, … - Development, 2002 - dev.biologists.org
... Factors important for the development of both primitive and definitive hematopoiesis
such as Scl, Lmo2, Gata1, Gata2 and Flk1 (Kdr) have been described (Pevny ...
Cited by 12 - Web Search - dev.biologists.org - ncbi.nlm.nih.gov

Myeloid lineage switch of Pax5 mutant but not wild-type B cell progenitors by C/EBPalpha and GATA … - Full text - MIT Libraries
B Heavey, C Charalambous, C Cobaleda, M Busslinger - EMBO J, 2003 - embojournal.npgjournals.com
... factors C/EBP , GATA1, GATA2 and GATA3 strongly promoted in vitro macrophage
differentiation and myeloid colony formation of Pax5 –/– pro-B cells. ...
Cited by 10 - Web Search - nature.com - pubmedcentral.nih.gov - ncbi.nlm.nih.gov - all 5 versions »

A nonsense mutation in zebrafish gata 1 causes the bloodless phenotype in vlad tepes - Full text - MIT Libraries
SE Lyons, ND Lawson, L Lei, PE Bennett, BM … - Proceedings of the National Academy of Sciences, 2002 - pnas.org
... levels of Gata2 were markedly elevated (20-fold) in a quantitative PCR analysis
(38), leading to the hypothesis that Gata2 may compensate for loss of Gata1. ...
Cited by 12 - Web Search - med.umich.edu - pubmedcentral.gov - dx.doi.org - all 8 versions »

Dcode. org anthology of comparative genomic tools
GG Loots, I Ovcharenko - nar.oxfordjournals.org
... Consistent with this hypothesis, it has been previously reported that in biochemical
assays GATA1 controls the expression of GATA2 transcript (39). ...
Web Search - pubmedcentral.nih.gov - ivan.dcode.org - citebase.eprints.org - all 5 versions »

Expression of alpha4-integrin defines the earliest precursor of hematopoietic cell lineage diverged … - Full text - MIT Libraries
M Ogawa, M Kizumoto, S Nishikawa, T Fujimoto, H … - Blood, 1999 - bloodjournal.org
... Different dilutions of cDNA prepared from sorted cells were subjected to PCR
amplification specific for Gata1, Gata2, Tal1, Lmo2, Myb, and Gapd transcripts. ...
Cited by 35 - Web Search - bloodjournal.org - ncbi.nlm.nih.gov

Molecular heterogeneity in AML/MDS patients with 3 q 21 q 26 rearrangements
I Lahortiga, I Vazquez, X Agirre, MJ Larrayoz, JL … - Genes Chromosomes and Cancer, 2004 - doi.wiley.com
... WGATAR (Orkin, 1992). Among these genes, GATA1, GATA2, and GATA3 play
crucial roles in hematopoiesis. GATA1 is highly expressed ...
Web Search - ncbi.nlm.nih.gov - Get it from MIT Libraries

The transcription factor GATA3 is a downstream effector of Hoxb1 specification in rhombomere 4 - Full text - MIT Libraries
I Pata, M Studer, JH van Doorninck, J Briscoe, S … - Development, 1999 - dev.biologists.org
... GATA2 appears to be required for proliferation of hematopoietic progenitor cells
and in primitive erythropoiesis, whereas GATA1 has a role in definitive ...
Cited by 31 - Web Search - dev.biologists.org - ncbi.nlm.nih.gov - ncbi.nlm.nih.gov

HrT is required for cardiovascular development in zebrafish - Full text - MIT Libraries
DP Szeto, KJ Griffin, D Kimelman - Development, 2002 - dev.biologists.org
... Secondly, we found that HrT is required to inhibit the expression of the blood lineage
markers gata1 and gata2 in the most posterior lateral plate mesoderm. ...
Cited by 14 - Web Search - dev.biologists.org - evolutionsbiologie.uni-konstanz.de - ncbi.nlm.nih.gov

Regulation of metallothionein gene transcription - Full text - MIT Libraries
LH Moilanen, T Fukushige, JH Freedman - J. Biol. Chem, 1999 - jbc.org
... Elements-- The ability of the C. elegans GATA-binding transcription factor ELT-2
to form complexes with GATA1.1, GATA2.1, or GATA2.4 elements was investigated. ...
Cited by 14 - Web Search - jbc.org - ncbi.nlm.nih.gov

Rapid turnover of GATA-2 via ubiquitin-proteasome protein degradation pathway - Full text - MIT Libraries
N Minegishi, N Suzuki, Y Kawatani, R Shimizu, M … - Genes to Cells, 2005 - blackwell-synergy.com
... A much more profound ablation of primitive hematopoiesis was found in Gata1 /y
::Gata2 / double knockout embryos than in Gata1 /y or Gata2 / single knockout ...
Web Search - ingentaconnect.com - genestocellsonline.org - ncbi.nlm.nih.gov

Transcriptional activity of TAL 1 in T cell acute lymphoblastic leukemia(T-ALL) requires RBTN 1 or-2 … - Full text - MIT Libraries
Y Ono, N Fukuhara, O Yoshie - J Biol Chem, 1997 - jbc.org
... TAL1 is considered to function in close collaboration with RBTN2 and a group of
GATA binding zinc finger type transcription factors, GATA1 and GATA2 (19). ...
Cited by 18 - Web Search - jbc.org - ncbi.nlm.nih.gov - ncbi.nlm.nih.gov

Loss of hspa9b in zebrafish recapitulates the ineffective hematopoiesis of the myelodysplastic … - Full text - MIT Libraries
SE Craven, D French, W Ye, F de Sauvage, A … - Blood, 2005 - bloodjournal.org
... 1C-F). However, overall blood development appeared normal up to 33 hpf as revealed
by whole-mount in situ hybridization for scl, gata2, and gata1 (Figure 1G-H ...
Web Search - bloodjournal.org - ncbi.nlm.nih.gov

Scl is required for dorsal aorta as well as blood formation in zebrafish embryos - Full text - MIT Libraries
LJ Patterson, M Gering, R Patient - Blood, 2005 - bloodjournal.org
... 10,50,51 Lmo2 and Gata2, activated before gata1 in the PLM, are likely partners
for Scl in this activation, although gain-of-function experiments show that ...
Web Search - bloodjournal.org - ncbi.nlm.nih.gov

A role for GATA5 in Xenopus endoderm specification - Full text - MIT Libraries
H Weber, CE Symes, ME Walmsley, AR Rodaway, RK … - Development, 2000 - dev.biologists.org
... is probably due to the conversion of ectoderm towards endoderm, the reason for the
downregulation of cytokeratin by GATA1 and 6 is still unclear. GATA2 and 3 ...
Cited by 37 - Web Search - dev.biologists.org - ncbi.nlm.nih.gov

Lineage analysis of the hemangioblast as defined by FLK 1 and SCL expression - Full text - MIT Libraries
YS Chung, WJ Zhang, E Arentson, PD Kingsley, J … - Development, 2002 - dev.biologists.org
... transferred to a nylon membrane, and hybridized with 32 P randomly primed cDNA probe
corresponding to the 3' end of the L32, Flk1, Scl, Gata1, Gata2 and Lmo2 ...
Cited by 28 - Web Search - dev.biologists.org - dx.doi.org - ncbi.nlm.nih.gov

The anemic Friend virus gp55 envelope protein induces erythroid differentiation in fetal liver … - Full text - MIT Libraries
SN Constantinescu, H Wu, X Liu, W Beyer, A Fallon, … - Blood, 1998 - bloodjournal.org
... A low level of expression of the GATA2 transcription factor (or a low GATA2/GATA1
ratio) was associated with erythroid differentiation and small-sized bursts. ...
Cited by 14 - Web Search - bloodjournal.org - ncbi.nlm.nih.gov

Ci-GATAa, aGATA-class gene from the ascidianCiona intestinalis: Isolation and developmental … - Full text - MIT Libraries
P D'Ambrosio, A Fanelli, M Pischetola, A Spagnuolo - Developmental Dynamics, 2003 - doi.wiley.com
... Members of the first group, GATA1, GATA2, GATA3, are implicated in the specification
of blood cell lineages (Pevny and Si- mon, 1991; Tsai et al., 1994 ...
Cited by 2 - Web Search - ncbi.nlm.nih.gov

The NFY Transcription Factor Inhibits von Willebrand Factor Promoter Activation in Non-endothelial … - Full text - MIT Libraries
Y Peng, N Jahroudi - J Biol Chem, 2003 - jbc.org
... FOG1 and FOG2 are examples of cofactors that regulate the function of GATA
transcription factor family members GATA1 and GATA2 (15, 16). ...
Cited by 5 - Web Search - jbc.org - ncbi.nlm.nih.gov