Myocardial activation of the human cardiac alpha-actin promoter by helix-loop-helix proteins. - Full text - MIT Libraries
V Sartorelli, NA Hong, NH Bishopric, L Kedes - Proc Natl Acad Sci US A, 1992 - pubmedcentral.nih.gov
... The same DNA sequence elements recognized by SRF, Sp1, and MyoD and required for
HCA transcription in skeletal muscle cells were also found to be necessary for ...
Cited by 31 - Web Search - pubmedcentral.nih.gov - ncbi.nlm.nih.gov

Specific inhibition of skeletal alpha-actin gene transcription by applied mechanical forces through … - Full text - MIT Libraries
AM Lew, M Glogauer, CA McUlloch - Biochem J, 1999 - biochemj.org
... TEF-1 and YY1 caused significant (P 0.01) increases in luciferase activity,
whereas SRF and SP1 caused signficant decreases (P 0.01). ...
Cited by 19 - View as HTML - Web Search - biochemj.org - ncbi.nlm.nih.gov - ncbi.nlm.nih.gov

Myogenic basic helix–loop–helix proteins and Sp1 interact as components of a multiprotein … - Full text - MIT Libraries
E Biesiada, Y Hamamori, L Kedes, V Sartorelli - Mol Cell Biol, 1999 - mcb.asm.org
... Thus, this protein complex appears to be formed in an all-or-none fashion with the
binding of SRF, Sp1, and myogenic bHLH proteins to the HCA promoter being ...
Cited by 35 - Web Search - mcb.asm.org - pubmedcentral.nih.gov - ncbi.nlm.nih.gov

Muscle actin genes: A first step towards computational classification of tissue specific promoters - Full text - MIT Libraries
K Frech, K Quandt, T Werner - In Silico Biology, 1998 - iospress.metapress.com
... SRF - TATA 0.005 0.245 1: 53.761 SRF - SP1 0.019 0.694 1: 35.712 MyoD -
MEF2 0.154 0.449 1: 2.925 CEBP - MyoD 0.167 0.449 1: 2.685 ...
Cited by 41 - Web Search - bioinfo.de - bioinfo.de - ncbi.nlm.nih.gov - all 5 versions »

Collaborative Roles for c-Jun N-terminal Kinase, c-Jun, Serum Response Factor, and Sp 1 in Calcium- … - Full text - MIT Libraries
PM McDonough, DS Hanford, AB Sprenkle, NR Mellon, … - J Biol Chem, 1997 - jbc.org
... gene expression that involves the activation of the MAPK family member JNK, the
proto-oncogene product c-Jun, and the transcriptional regulators SRF and Sp1. ...
Cited by 39 - Web Search - jbc.org - ncbi.nlm.nih.gov - ncbi.nlm.nih.gov

Organization and myogenic restricted expression of the murine serum response factor gene - Full text - MIT Libraries
NS Belaguli, LA Schildmeyer, RJ Schwartz - J. Biol. Chem, 1997 - jbc.org
... A and B show binding of SRF and Sp1 from myotube nuclear extracts to cognate probes. ...
Sp1 Binding Activity Is Required for SRF Promoter Activity ...
Cited by 61 - Web Search - jbc.org - ncbi.nlm.nih.gov

Expression of the serum response factor gene is regulated by serum response factor binding sites. - Full text - MIT Libraries
JA Spencer, RP Misra - J Biol Chem, 1996 - jbc.org
... boxes are not sufficient to mediate serum stimulation of the SRF promoter, these
results suggest that in the context of an intact SRF promoter Sp1 may interact ...
Cited by 37 - Web Search - jbc.org - ncbi.nlm.nih.gov

Interaction of CArG Elements and a GC-rich Repressor Element in Transcriptional Regulation of the … - Full text - MIT Libraries
CS Madsen, CP Regan, GK Owens - J Biol Chem, 1997 - jbc.org
... Values of p < 0.05 were considered statistically significant. Preparation of
Recombinant SRF, Sp1, and Electrophoretic Mobility Shift Assays (EMSAs) ...
Cited by 24 - Web Search - jbc.org - ncbi.nlm.nih.gov - ncbi.nlm.nih.gov

O glycosylation of an Sp1-derived peptide blocks known Sp1 protein interactions - Full text - MIT Libraries
MD Roos, K Su, JR Baker, JE Kudlow - Mol. Cell. Biol, 1997 - mcb.asm.org
... activation domain to which an O-GlcNAc site was mapped (26). As shown in
Fig. 1, 7 of 9 amino acids in the SRF sequence match Sp1. ...
Cited by 64 - Web Search - mcb.asm.org - pubmedcentral.nih.gov - ncbi.nlm.nih.gov - all 5 versions »

Rho GTPases as Modulators of the Estrogen Receptor Transcriptional Response - Full text - MIT Libraries
LF Su, R Knoblauch, MJ Garabedian - J Biol Chem, 2001 - jbc.org
... Fig. 4. Rho GDI inhibits transcriptional activation by SRF and Sp1. U2OS
cells were transfected as in Fig. 2 with 0.4 µg of Rho ...
Cited by 21 - Web Search - jbc.org - ncbi.nlm.nih.gov - ncbi.nlm.nih.gov

Eukaryotic Regulatory Element Conservation Analysis and Identification Using Comparative Genomics - Full text - MIT Libraries
Y Liu, XS Liu, L Wei, RB Altman, S Batzoglou - Genome Research, 2004 - genome.org
... 2000). Known regulatory elements in this data set include binding sites for
tran- scription factors Mef2, Myf, Srf, Sp1, Tef, and Nvl. ...
Cited by 16 - Web Search - gremlin1.zool.iastate.edu - ai.stanford.edu - dx.doi.org - all 13 versions »

Muscle-specific expression of the cardiac alpha-actin gene requires MyoD1, CArG-box binding factor, …
V Sartorelli, KA Webster, L Kedes - Genes Dev, 1990 - genesdev.org
... Previously characterized sites interact with a protein identical with or related
to the serum response factor (SRF) at -100 and Sp1 at -70. ...
Cited by 78 - Web Search - ncbi.nlm.nih.gov - Get it from MIT Libraries

Collaborative interactions between MEF‐2 and Sp 1 in muscle‐specific gene regulation - Full text - MIT Libraries
J Grayson, R Bassel-Duby, RS Williams - Journal of Cellular Biochemistry, 1998 - doi.wiley.com
... of fusion to heterologous protein segments, GDB- MEF2C(1–117) in combination with
GAD, GDB- SRF(144–235) in combination with GAD or with GAD-Sp1(1–621 ...
Cited by 16 - Web Search - doi.wiley.com - ncbi.nlm.nih.gov

Sp1 represses IL-2 receptor alpha chain gene expression
DG Roman, MB Toledano, WJ Leonard - New Biol, 1990 - ncbi.nlm.nih.gov
... expression. In this study, the results of competition experiments suggest that
Sp1 and SRF compete for binding to the CArG region. Site ...
Cited by 10 - Web Search - Get it from MIT Libraries

Role of transcription factor TFIIF in serum response factor-activated transcription - Full text - MIT Libraries
H Zhu, V Joliot, R Prywes - J Biol Chem, 1994 - jbc.org
... A low amount of TFIIF was sufficient for basal transcription, whereas higher
amounts were required for SRF, but not Sp1, activation. ...
Cited by 25 - Web Search - ncbi.nlm.nih.gov - ncbi.nlm.nih.gov

A serum response factor-dependent transcriptional regulatory program identifies distinct smooth … - Full text - MIT Libraries
S Kim, HS Ip, MM Lu, C Clendenin, MS Parmacek - Mol Cell Biol, 1997 - mcb.asm.org
... reporter plasmid. A mutation in the SME-1 site that completely abolished binding
of SRF and Sp1 (complexes A to C in Fig. 4A) resulted ...
Cited by 108 - Web Search - uphs.upenn.edu - pubmedcentral.nih.gov - ncbi.nlm.nih.gov - all 6 versions »

An approach to identify over-represented cis-elements in related sequences - Full text - MIT Libraries
J Zheng, J Wu, Z Sun - Nucleic Acids Research, 2003 - nar.oupjournals.org
... For the putative transcription factor binding sites located in actin promoters,
SRF and Sp1 were previously reported in many articles to perform functional ...
Cited by 7 - Web Search - pubmedcentral.nih.gov - ingentaconnect.com - ncbi.nlm.nih.gov - all 7 versions »

The anti-cancer agent distamycin A displaces essential transcription factors and selectively …
A Taylor, KA Webster, TA Gustafson, L Kedes - Mol. Cell. Biochem, 1997 - springerlink.com
... The SRF, Sp1 and MyoD binding site sequences correspond to regu- latory sites
previously characterized within the human car- diac α-actin promoter [23]. ...
Cited by 10 - Web Search - kluweronline.com - ncbi.nlm.nih.gov - Get it from MIT Libraries

Calcium-regulated changes in mitochondrial phenotype in skeletal muscle cells - Full text - MIT Libraries
D Freyssenet, I Irrcher, MK Connor, M Di Carlo, DA … - Am J Physiol Cell Physiol, 2004 - intl-ajpcell.physiology.org
... A23187 also increased Egr-1, SRF and Sp1 protein expression, ... effect of A23187 on
SRF and Sp1 expression was completely dissipated by 48 hours (Fig. 4A, B). ...
Cited by 4 - Web Search - intl-ajpcell.physiology.org - ajpcell.physiology.org - ncbi.nlm.nih.gov - all 5 versions »

… granulosa cells by FSH and LH; combinatorial regulation by transcription factors CREB, SRF, Sp1, and … - Full text - MIT Libraries
DL Russell, KMH Doyle, I Gonzales-Robayna, C … - Molecular Endocrinology, 2003 - mend.endojournals.org
... Egr-1 induction in rat granulosa cells by FSH and LH; combinatorial regulation
by transcription factors CREB, SRF, Sp1, and Egr-1. ...
Cited by 1 - Web Search

Transforming growth factor-ß signal transduction - Full text - MIT Libraries
T Brand, MD Schneider - Circ Res, 1996 - circresaha.org
... Basal transcription of the promoter in cardiac myocytes required the MADS box protein
SRF, Sp1, and TEF-1 in concert, reminiscent of the need for SRF, Sp1, and ...
Cited by 13 - Web Search - circres.ahajournals.org - intl-circres.ahajournals.org - ncbi.nlm.nih.gov

Decoding Human Regulatory Circuits - Full text - MIT Libraries
W Thompson, MJ Palumbo, WW Wasserman, JS Liu, CE … - Genome Research, 2004 - genome.org
... Using the default parameters and matrices derived from the reported Myf, Mef-2,
SRF, SP1, and Tef aligned pairs of sites, COMET, at an E-value cutoff of 1.0 ...
Cited by 2 - Web Search - people.fas.harvard.edu - pubmedcentral.nih.gov - dx.doi.org - all 7 versions »

AG/C Element Mediates Repression of the SM22 {alpha} Promoter Within Phenotypically Modulated Smooth … - Full text - MIT Libraries
BR Wamhoff, MH Hoofnagle, A Burns, S Sinha, OG … - Circulation Research, 2004 - circresaha.org
... ChIP assays 8 were thus used to determine the effect of PDGF-BB treatment on SRF
and Sp1 enrichment of the CArG2–G/C–CArG1 motif in the SM22 or SMMHC ...
Cited by 2 - Cached - Web Search - circres.ahajournals.org - dx.doi.org - ncbi.nlm.nih.gov - all 8 versions »

Basic fibroblast growth factor activates serum response factor gene expression by multiple distinct … - Full text - MIT Libraries
JA Spencer, ML Major, RP Misra - Mol Cell Biol, 1999 - mcb.asm.org
... not shown). Another possibility is that Sp factors are permissive for
a complex which consists of at least SRF and Sp1. In this ...
Cited by 9 - Web Search - pubmedcentral.nih.gov - mcb.asm.org - ncbi.nlm.nih.gov - all 5 versions »

Binding of Serum Response Factor to CArG Box Sequences Is Necessary but Not Sufficient to Restrict … - Full text - MIT Libraries
M Strobeck, S Kim, JCL Zhang, C Clendenin, KL Du, … - J Biol Chem, 2001 - jbc.org
... to p-441SM22.LacZ except three nucleotides (underlined) in SME-1 (5'-
TCCTGCCCATAGATCTTTTTTCCC-3') were mutated to abolish SRF and Sp1 binding activity ( ...
Cited by 29 - Web Search - jbc.org - uphs.upenn.edu - ncbi.nlm.nih.gov

Regulation of Egr-1, SRF, and Sp1 mRNA expression in contracting skeletal muscle cells - Full text - MIT Libraries
I Irrcher, DA Hood - J Appl Physiol, 2004 - jap.org
... Regulation of Egr-1, SRF, and Sp1 mRNA expression in contracting skeletal muscle
cells. ... Effects of time and recovery on Egr-1, Sp1, and SRF mRNA expression. ...
Cached - Web Search - jap.physiology.org - jap.physiology.org - ncbi.nlm.nih.gov - all 5 versions »

Electrical activity regulates AChR gene expression via JNK, PKCj and Sp1 in skeletal chick muscle
N Altiok, JP Changeux - pasteur.fr
... has been shown that activation of the human car- diac K-actin promoter in skeletal
muscle requires the integrity of DNA binding sites for the SRF, Sp1 and for ...
View as HTML - Web Search

Identifying synonymous regulatory elements in vertebrate genomes
CR Guidelines, A by Ovcharenko, IA by Nobrega - nar.oxfordjournals.org
... version (100K): [in this window] [in a new window], Figure 2 GNF Expression Atlas2
analysis for genes identified in the SRF/SP1 SynoR scan of the human genome. ...
Web Search - nar.oxfordjournals.org

Composite regulatory elements: structure, function and classification.
S Receptor, HMG NF-kB, ETS SRF c-fos - Regulation - gene-regulation.com
... C00185. CEs containing binding sites for ETS and MADS factors. ETS, SRF, c-fos,
human egr-1, mouse. ... Oct-2A, lymphoid-specific. Sp1, ubiquitous Oct-1, ubiquitous. ...
Cached - Web Search - Get it from MIT Libraries

In Silico Identification of Regulatory Elements of GRIN 1 Genes
MK Mejia-Guerra, LR Lareo - Omics A Journal of Integrative Biology, 2005 - dx.doi.org
... Position 800 to 700 Lyf-1, Sp1, SRF SRF, Kr MEF-2 En, Sp1, GCF, ANF, ... Sp1 ODC1, (4
copies), RXR , Olf-1 XPF-1 400 to 300 Sp1 D1, SRF, Sp1 SRF, SRF, Sp1 SRF, ...
Web Search - liebertonline.com - liebertonline.com - ncbi.nlm.nih.gov - Get it from MIT Libraries

In silico Prediction of the Transcriptional Regulation of Human Genes
Z Weng - jax.org
... Muscle specific regulatory regions Cis-elements: Mef-2 Myf SRF Tef Sp1 (Data taken
from Wasserman and Fickett 1998) Page 40. Measures of Performance ...
View as HTML - Web Search

Identifying synonymous regulatory elements in vertebrate genomes
I Ovcharenko, MA Nobrega - seeds - pubmedcentral.nih.gov
... We applied SynoR to predict targets of synergistic SRF/SP1 gene regulation in the
human genome using as a seed motif the presence of these TFBS separated by ...
Web Search - nar.oxfordjournals.org - ivan.dcode.org - citebase.eprints.org

Induction of early growth-response factor 1 by platelet-derived growth factor in human airway smooth … - Full text - MIT Libraries
J Hjoberg, L Le, A Imrich, V Subramaniam, SI … - Am J Physiol Lung Cell Mol Physiol, 2004 - intl-ajplung.physiology.org
... Coincubation with antibodies to SRF and SP1 also produced supershifted band
(arrows). ... SRF/ELK/SP1 complexes bind to the EGR-1 promoter. ...
Cited by 4 - Web Search - intl-ajplung.physiology.org - ncbi.nlm.nih.gov - ncbi.nlm.nih.gov

Differential patterns of human cytomegalovirus gene expression in various T-cell lines carrying …
Z Beck, A Bacsi, X Liu, P Ebbesen, I Andirko, E … - Journal of Medical Virology, 2003 - doi.wiley.com
... 5B). SRF and SP1 were also identified by supershift analysis (Fig. 5C,D). Effect
of SP1 Overexpression on HCMV Replication in MT-2 and MT-4 Cells ...
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M-CAT, CarG and Sp1 elements are required for 1-adrenergic induction of the skeletal-actin promoter … - Full text - MIT Libraries
LR Karns, K Kariya, PC Simpson - J. Biol. Chem, 1995 - jbc.org
... target for -adrenergic signaling at the skACT promoter, or whether the SRF is simply ...
promoter fragment (-155/-76) containing a CArG and a putative Sp1 site did ...
Cited by 8 - Web Search - jbc.org

Smooth Muscle-Specific Transcription Without a CArG Box Element - Full text - MIT Libraries
MW Majesky - Circulation Research, 2002 - circres.ahajournals.org
... of the cardiac -actin promoter in skeletal muscle cells was shown to depend upon
a muscle-specific multiprotein complex containing myogenin, Sp1, and SRF. ...
Cited by 1 - Web Search - circres.ahajournals.org - ahavj.ahajournals.org - ncbi.nlm.nih.gov

Regulation of MCL 1 through a Serum Response Factor/Elk-1-mediated Mechanism Links Expression of a … - Full text - MIT Libraries
KJ Townsend, P Zhou, L Qian, CK Bieszczad, CH … - J Biol Chem, 1999 - dx.doi.org
... Serum response factor (SRF), Elk-1, and Sp1 bound to cognate sites within this segment,
SRF and Elk-1 acting coordinately to affect both basal activity and TPA ...
Cited by 31 - Web Search - jbc.org - jbc.org - ncbi.nlm.nih.gov - all 5 versions »

Interaction with RAP 74 subunit of TFIIF is required for transcriptional activation by serum … - Full text - MIT Libraries
V Joliot, M Demma, R Prywes - Nature, 1995 - nature.com
... to support SRF-activated transcription in vitro but has little effect on the protein's
basal transcription activity or its ability to support SP1-activated ...
Cited by 45 - Web Search - ncbi.nlm.nih.gov - ncbi.nlm.nih.gov

Interaction of the v-Rel oncoprotein with cellular transcription factor Sp1 - Full text - MIT Libraries
S Sif, TD Gilmore - J. Virol, 1994 - pubmedcentral.nih.gov
... The v-Rel oncoprotein increases expression from Sp1 site-containing promoters in
chicken ... binds to NF-kappa B p50 and serum response factor (SRF) and associates ...
Cited by 20 - Web Search - pubmedcentral.nih.gov - jvi.asm.org - ncbi.nlm.nih.gov

p21WAF1/CIP1 is upregulated by the geranylgeranyltransferase I inhibitor GGTI-298 through a … - Full text - MIT Libraries
J Adnane, FA Bizouarn, Y Qian, AD Hamilton, SM … - Mol. Cell. Biol, 1998 - mcb.asm.org
... and Sp1-Responsive Element: Involvement ... Electrophoretic mobility shift assays showed
a small increase in the amount of DNA-bound Sp1-Sp3 complexes. ...
Cited by 64 - Web Search - mcb.asm.org - pubmedcentral.nih.gov - ncbi.nlm.nih.gov

Role of Sp1 in insulin regulation of gene expression
SL Samson, NC Wong - J. Mol. Endocrinol, 2002 - journals.endocrinology.org
... Solomon SS, Palazzo MR, Takahashi T & Raghow R. 1997 Transcription factor Sp1 is
necessary ... 1999 Expression of the SRF gene occurs through a Ras/Sp/SRF-mediated ...
Cited by 14 - Cached - Web Search - dx.doi.org - jme.endocrinology-journals.org - ncbi.nlm.nih.gov - all 5 versions » - Get it from MIT Libraries

Sp1 and Sp3 physically interact and co-operate with GABP for the activation of the utrophin promoter - Full text - MIT Libraries
F Galvagni, S Capo, S Oliviero - J. Mol. Biol, 2001 - unisi.it
... by adding speci®c antibody for Sp1 (lanes 3, 7 and 11) or Sp3 (lanes 4, 8 and 12)
to the reaction mixture. The unrelated (unr) antibody anti-SRF did not ...
Cited by 24 - Web Search - unisi.it - ncbi.nlm.nih.gov - all 4 versions »

SRF protein is upregulated during stretch-induced hypertrophy of rooster ALD muscle - Full text - MIT Libraries
M Flueck, JA Carson, RJ Schwartz, FW Booth - J Appl Physiol, 1999 - jap.org
... Home page I. Irrcher and DA Hood Regulation of Egr-1, SRF, and Sp1 mRNA expression
in contracting skeletal muscle cells J Appl Physiol, December 1, 2004; 97(6 ...
Cited by 19 - Cached - Web Search - jap.physiology.org - jap.org - ncbi.nlm.nih.gov - all 5 versions »

NAB2: A transcriptional brake for activated gene expression in the vessel wall - Full text - MIT Libraries
JM Miano, BC Berk - Am. J. Pathol, 1999 - ajp.amjpathol.org
... 10 The 5' flanking region of SRF contains overlapping sites for Sp1 and Egr-1. 12
Thus, the transient nature of SRF-dependent IEG activation may also be due to ...
Cited by 8 - Web Search - ncbi.nlm.nih.gov

Induction of vascular smooth muscle alpha-actin gene transcription in transforming growth factor … - Full text - MIT Libraries
SV Subramanian, JA Polikandriotis, RJ Kelm Jr, JJ … - Mol. Biol. Cell, 2004 - pubmedcentral.nih.gov
... may be related to differences in the levels of native Pur proteins and/or availability
of SMA enhancer activating factors such as Sp1, TEF1, or SRF in each ...
Cited by 2 - Web Search - ijmm.org - ncbi.nlm.nih.gov

Regulation of SRF Activity by Rho Family GTPases
R TREISMAN, AS ALBERTS, E SAHAI - Cold Spring Harbor Symposia on Quantitative Biology, 1998 - dx.doi.org
... 1994) and cooperate with the SRF carboxy-terminal activation domain to activate
transcription (Hill et al. ... Regulation of SRF Activity by Rho Family GTPases ...
Cited by 21 - Web Search - cshl-symposium.org - cshl-symposium.org - ncbi.nlm.nih.gov - all 5 versions » - Get it from MIT Libraries

Transcription factor Sp1 expression is a significant predictor of survival in human gastric cancer - Full text - MIT Libraries
L Wang, D Wei, S Huang, Z Peng, X Le, TT Wu, J Yao … - Clin Cancer Res, 2003 - intl-clincancerres.aacrjournals.org
... [Abstract/Free Full Text]; Sorensen P., Wintersberger E. Sp1 and NF-Y ... [Abstract/Free
Full Text]; Spencer JA, Misra RP Expression of the SRF gene occurs through a ...
Cited by 11 - Web Search - intl-clincancerres.aacrjournals.org - ncbi.nlm.nih.gov

… transcriptional coactivator for serum response factor (SRF), is required for serum induction of SRF … - Full text - MIT Libraries
B Cen, A Selvaraj, RC Burgess, JK Hitzler, Z Ma, … - Mol Cell Biol, 2003 - mcb.asm.org
... In addition, SRF binds and functionally cooperates with several other transcriptional
factors, including Sp1, C/EBPß, and TFII-I (24, 33, 52, 54). ...
Cited by 15 - Web Search - pubmedcentral.nih.gov - columbia.edu - dx.doi.org - all 7 versions »

Induction of Vascular Smooth Muscle {alpha}-Actin Gene Transcription in Transforming Growth Factor { …
M Editor, CH Heldin - molbiolcell.org
... may be related to differences in the levels of native Pur proteins and/or availability
of SMA enhancer activating factors such as Sp1, TEF1, or SRF in each ...
Web Search - molbiolcell.org

Prediction of C regulatory elements using statistical analysis.
P Qiu, W Ding, Y Jiang, J Greene, L Wang - ScientificWorldJournal, 2002 - thescientificworld.com
... By this approach, we have been able to Page 2. confirm quite some, for example
Pbx and Oct-1, C/EBP and SRF, YY1 and NF-AT, EGR and SP1 etc. ...
Web Search - ncbi.nlm.nih.gov - Get it from MIT Libraries