1: Biol Reprod. 2005 May;72(5):1194-204. Epub 2005 Jan 12. 

Expression of CCAAT/enhancer binding proteins alpha and beta in the porcine
ovary and regulation in primary cultures of granulosa cells.

Gillio-Meina C, Hui YY, LaVoie HA.

Department of Cell and Developmental Biology and Anatomy, University of South
Carolina School of Medicine, Columbia, South Carolina 29208, USA.

CCAAT/enhancer binding proteins alpha and beta (CEBPA/ CEBPB) were evaluated in
the porcine ovary during the estrous cycle. CEBPB mRNA was present in antral
follicles and was significantly increased in healthy corpora lutea (CL), whereas
CEBPA mRNA was constitutively expressed in these structures. Both isoforms of
CEBPA (42 and 30 kDa) exhibited greater expression in preovulatory follicles,
and the 42-kDa isoform increased in CL, whereas the 30-kDa isoform decreased.
All major isoforms of CEBPB (38, 34, and 20 kDa) were expressed, with the 34-
and 20-kDa isoforms being more abundant in preovulatory follicles and further
increased in CL. The effects of FSH and cAMP analogue on the distribution of
CEBP isoforms were evaluated in primary cultures of porcine granulosa cells. FSH
and 8-Br-cAMP had little stimulatory effect on isoform distribution, but cAMP
treatment for 24 h tended to decrease the 30-kDa form of CEBPA and the 34-kDa
form of CEBPB. The 34-kDa form of CEBPB was decreased by the protein kinase A
inhibitor H89 at 4 h (with FSH treatment), and by both protein kinase A and
phosphatidylinositol 3-kinase inhibitors at 24 h of treatment. In transfected
granulosa cells, FSH and cAMP analogue stimulated a CEBP consensus
sequence-reporter construct that was blocked by H89. These data implicate
protein kinase A as the major regulator of CEBPB isoform distribution and
CEBP-mediated transactivation in granulosa cells. The differential expression of
specific CEBPA/B isoforms observed in maturing follicles and CL may contribute
to changes in follicular cell differentiation and increasing steroidogenic
capacity.

PMID: 15647458 [PubMed - indexed for MEDLINE]


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2: Genomics. 1995 Jul 20;28(2):333-6. 

Mouse chromosomal location of the CCAAT/enhancer binding proteins C/EBP beta
(Cebpb), C/EBP delta (Cebpd), and CRP1 (Cebpe).

Jenkins NA, Gilbert DJ, Cho BC, Strobel MC, Williams SC, Copeland NG, Johnson
PF.

ABL-Basic Research Program, NCI-Frederick Cancer Research and Development
Center, Maryland 21702-1201, USA.

There are four known members of the C/EBP family of basic region-leucine zipper
transcription factors: Cebpa, Cebpb, Cebpd, and Cebpe. Cebpa has previously been
mapped to mouse chromosome 7. Here, we show that Cebpb maps to mouse chromosome
2, Cebpd to chromosome 16, and Cebpe to chromosome 14. The assignment of Cebpd
to chromosome 16 identifies a new region of homology between mouse chromosome 16
and human chromosome 8.

PMID: 8530045 [PubMed - indexed for MEDLINE]


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3: Genomics. 1992 Sep;14(1):12-7. 

The CCAAT/enhancer binding protein (C/EBP alpha) gene (CEBPA) maps to human
chromosome 19q13.1 and the related nuclear factor NF-IL6 (C/EBP beta) gene
(CEBPB) maps to human chromosome 20q13.1.

Hendricks-Taylor LR, Bachinski LL, Siciliano MJ, Fertitta A, Trask B, de Jong
PJ, Ledbetter DH, Darlington GJ.

Institute for Molecular Genetics, Baylor College of Medicine, Houston, Texas
77054.

The CEBPA gene encoding CCAAT/enhancer binding protein (C/EBP alpha) has been
mapped to human chromosome 19 and the CEBPB (formerly TCF5) gene encoding NF-IL6
(C/EBP beta) to human chromosome 20 by Southern blot analysis of Chinese hamster
x human and mouse x human somatic cell hybrids. CEBPA has been further mapped to
19q13.1 between the loci GPI and TGFB using human x hamster somatic cell hybrids
containing restricted fragments of human chromosome 19. This position was
confirmed by fluorescence in situ hybridization. Furthermore, CEBPB has been
mapped to 20q13.1 by fluorescence in situ hybridization.

PMID: 1427819 [PubMed - indexed for MEDLINE]


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