1: Biochem Biophys Res Commun. 1999 Nov 2;264(3):668-75. 

Involvement of the Sp1 site in ras-mediated downregulation of the RECK
metastasis suppressor gene.

Sasahara RM, Takahashi C, Noda M.

Department of Molecular Oncology, Kyoto University Graduate School of Medicine,
Yoshida-Konoe-cho, Sakyo-ku, Kyoto, 606-8501, Japan.

We have isolated and characterized the 5'-flanking region of the mouse RECK gene
aiming to understand the mechanism of oncogene-mediated suppression of RECK gene
expression. The upstream 52-base region was found to contain a promoter activity
which is, to some extent, suppressed by the ras oncogene. This region contains
two Sp1-binding motifs, one cEBPb-binding motif, and one CAAT box. Although both
of the Sp1 sites were found to associate with Sp1 as well as Sp3 proteins, ras
responsiveness seems to be mediated only by the downstream Sp1 site. Our data
indicate that the Sp1 motif in certain contexts can serve as a negative target
for the Ras signal. Copyright 1999 Academic Press.

PMID: 10543990 [PubMed - indexed for MEDLINE]


---------------------------------------------------------------