1: Cancer Res. 2002 Jun 1;62(11):2993-8. 

Microarray-based copy number and expression profiling in dedifferentiated and
pleomorphic liposarcoma.

Fritz B, Schubert F, Wrobel G, Schwaenen C, Wessendorf S, Nessling M, Korz C,
Rieker RJ, Montgomery K, Kucherlapati R, Mechtersheimer G, Eils R, Joos S,
Lichter P.

Deutsches Krebsforschungszentrum, Abteilung Molekulare Genetik (H0700), Im
Neuenheimer Feld 280, D-69120 Heidelberg, Germany.

Sixteen dedifferentiated and pleomorphic liposarcomas were analyzed by
comparative genomic hybridization (CGH) to genomic microarrays (matrix-CGH),
cDNA-derived microarrays for expression profiling, and by quantitative PCR.
Matrix-CGH revealed copy number gains of numerous oncogenes, i.e., CCND1, MDM2,
GLI, CDK4, MYB, ESR1, and AIB1, several of which correlate with a high level of
transcripts from the respective gene. In addition, a number of genes were found
differentially expressed in dedifferentiated and pleomorphic liposarcomas.
Application of dedicated clustering algorithms revealed that both tumor subtypes
are clearly separated by the genomic profiles but only with a lesser power by
the expression profiles. Using a support vector machine, a subset of five clones
was identified as "class discriminators." Thus, for the distinction of these
types of liposarcomas, genomic profiling appears to be more advantageous than
RNA expression analysis.

PMID: 12036902 [PubMed - indexed for MEDLINE]


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