1: Gene. 2000 Dec 30;260(1-2):103-12. 

Genomic organization, 5'flanking region and tissue-specific expression of mouse
phosphofructokinase C gene.

Gunasekera D, Kemp RG.

Department of Biochemistry and Molecular Biology, Chicago Medical School, 3333
Green Bay Road, North Chicago, IL 60064, USA.

Using a combination of mouse bacterial artificial chromosome (BAC) genomic
library screening, long-range polymerase chain reaction (PCR) amplification,
genomic walking and DNA sequencing, we have characterized the intron/exon
boundaries, the sizes of each intron and 5' flanking region of the mouse PFK-C
gene. The gene spans approximately 55 kb and comprises 22 exons separated by 21
introns. All intron/exon splice junctions conform to the GT/AG rule. The mouse
PFK-C gene organization is similar to that of the human and rabbit PFK-A and
human and mouse PFK-B genes. However, PFK-C has much larger intronic sequences
throughout the gene. Anchored PCR was performed to amplify about 1.0 kb of
genomic DNA upstream of the translational start site. Sequence analysis of the
PFK-C 5' flanking region revealed that it is devoid of TATA and CAAT boxes at
the usual positions, but it contained several putative binding sites for
transcription factors AP1, GATA1, NKX2.5 and STAT. The 5' flanking region was
not enriched in GC dinucleotides and lacked CpG islands and putative binding
sites for SP1. Four transcription initiation sites have been identified by
full-length RNA ligase-mediated rapid amplification of cDNA ends (RLM-RACE)
between -61 and -32 bp from the translation initiation codon.Reverse
transcription-PCR analysis revealed that PFK-A, PFK-B and PFK-C genes were
expressed, in all mouse tissues tested, at varying levels. PFK-A mRNA was more
abundantly expressed in all tissues than were the PFK-B and PFK-C genes. Based
on the mouse PFK-C signal normalized to 18S rRNA, the PFK-C mRNA was expressed
at the highest levels in the brain, heart, thymus and testicles, whereas low
levels were observed in the kidney, liver, muscle, and lung.

PMID: 11137296 [PubMed - indexed for MEDLINE]


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