1: Nucleic Acids Res. 1991 Apr 11;19(7):1413-9. 

Hypersensitive site 4 of the human beta globin locus control region.

Pruzina S, Hanscombe O, Whyatt D, Grosveld F, Philipsen S.

National Institute for Medical Research, Mill Hill, London, UK.

The Locus Control Region (LCR) of the human beta globin gene domain is defined
by four erythroid-specific DNasel hypersensitive sites (HSS) located upstream of
this multigene cluster. The LCR confers copy number dependent high levels of
erythroid specific expression to a linked transgene, independent of the site of
integration. To assess the role of the individual hypersensitive sites of the
LCR, we have localized HSS4 to a 280bp fragment that is functional both in
murine erythroleukaemia (MEL) cells and in transgenic mice. This fragment
coincides with the major area of hypersensitivity 'in vivo' and contains a
number of DNasel footprints. Bandshift analysis shows that these footprints
correspond to binding sites for the erythroid specific proteins GATA1 and NF-E2
and a number of ubiquitous proteins, including jun/fos, Sp1 and TEF2.

PMID: 2027748 [PubMed - indexed for MEDLINE]


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