1: Proc Natl Acad Sci U S A. 1994 May 10;91(10):4372-6. 

SRY protein enhances transcription of Fos-related antigen 1 promoter constructs.

Cohen DR, Sinclair AH, McGovern JD.

Division of Cell Biology, John Curtin School of Medical Research, Australian
National University, Canberra.

In mammals, testis determination is under the control of the chromosome Y-linked
SRY gene. Sry is expressed in the fetal mouse just before development of the
testis and shows germ-cell-dependent expression in the adult mouse. SRY protein
contains a high-mobility-group (HMG)-box DNA-binding domain, and potential
target sequences have been identified. The fos-related antigen 1 (fra-1) gene is
closely related to the protooncogene c-fos and encodes a component of
transcription factor AP-1. Fra-1 is expressed during spermatogenesis, and the
promoter of the rat fra-1 gene contains several potential binding sites for
members of the HMG-box family of DNA-binding proteins. We demonstrate that
purified SRY protein binds strongly to one of the putative fra-1 HMG-box
response elements and that SRY enhances the transcription of rat fra-1 promoter
constructs in cotransfection experiments. These results suggest that the
function of HMG-box transcription factors may be mediated, in part, by
activation of members of the AP-1 transcription factor family.

PMID: 8183916 [PubMed - indexed for MEDLINE]


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