1: Genes Chromosomes Cancer. 2005 Feb;42(2):170-8. 

Activation of HLXB9 by juxtaposition with MYB via formation of t(6;7)(q23;q36)
in an AML-M4 cell line (GDM-1).

Nagel S, Kaufmann M, Scherr M, Drexler HG, MacLeod RA.

DSMZ-German Collection of Microorganisms and Cell Cultures, Department of Human
and Animal Cell Cultures, Braunschweig, Germany.

Mutation or dysregulation of related homeobox genes occurs in leukemia. Using
RT-PCR, we screened members of the EHG family of homeobox genes, comprising EN1
(at 2q14), GBX2 (at 2q36), and EN2, GBX1, and HLXB9 (at 7q36), for dysregulation
in acute myeloid leukemia (AML) cell lines indicated by chromosomal breakpoints
at these sites. Only one EHG-family gene was expressed, HLXB9, in cell line
GDM-1 (AML-M4). Karyotypic analysis of GDM-1 revealed a unique t(6;7)(q23;q35),
also present in the patient. Fluorescence in situ hybridization analysis showed
chromosomal breakpoints close to the region upstream of HLXB9, at 7q36, a region
rearranged in certain AML patients, and at 6q23 upstream of MYB, a gene
activated in leukemia. Detailed expression analysis suggested ectopic activation
of HLXB9 occurred via juxtaposition with regions upstream of MYB, which was
highly expressed in GDM-1. Our data identified a cell line model for a novel
leukemic translocation involving MYB with HLXB9, further implicating HLXB9 in
leukemogenesis. (c) 2004 Wiley-Liss, Inc.

PMID: 15540222 [PubMed - indexed for MEDLINE]


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