1: Mol Cell Biol. 1990 Oct;10(10):5226-34. 

REB1, a yeast DNA-binding protein with many targets, is essential for growth and
bears some resemblance to the oncogene myb.

Ju QD, Morrow BE, Warner JR.

Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York
10461.

REB1 is a DNA-binding protein that recognizes sites within both the enhancer and
the promoter of rRNA transcription as well as upstream of many genes transcribed
by RNA polymerase II. We report here the cloning of the gene for REB1 by
screening a yeast genomic lambda gt11 library with specific oligonucleotides
containing the REB1 binding site consensus sequence. The REB1 gene was
sequenced, revealing an open reading frame encoding 809 amino acids. The
predicted protein was highly hydrophilic, with numerous OH-containing amino
acids and glutamines, features common to many of the general DNA-binding
proteins of Saccharomyces cerevisiae, such as ABF1, RAP1, GCN4, and HSF1. There
was some homology between a portion of REB1 and the DNA-binding domain of the
oncogene myb. REB1 is an essential gene that maps on chromosome II. However, the
physiological role that it plays in the cell has yet to be established.

PMID: 2204808 [PubMed - indexed for MEDLINE]


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