1: Lung Cancer. 2002 Dec;38(3):229-34. 

The promoter region of the human BUBR1 gene and its expression analysis in lung
cancer.

Seike M, Gemma A, Hosoya Y, Hosomi Y, Okano T, Kurimoto F, Uematsu K, Takenaka
K, Yoshimura A, Shibuya M, Ui-Tei K, Kudoh S.

Fourth Department of Internal Medicine, Nippon Medical School, 1-1-5 Sendagi,
Bunkyo-ku, Tokyo 113-8602, Japan.

Mitotic checkpoint impairment is present in human lung cancers with chromosomal
instability (CIN). Spindle-checkpoint genes have been reported to be mutated in
several human cancers, but these mutations are infrequent. Recent reports
suggest that the hBUBR1 gene may play an important role in mitotic checkpoint
control and in mitotic checkpoint impairment in human cancers. We analyzed the
expression of hBUBR1 in lung cancer cell lines using real time quantitative
RT-PCR. The expression of BUBR1 was found to be up-regulated in all of these
cell lines. In addition, we cloned and characterized the promotor region of
hBUBR1 and determined its genomic structure, which includes 23 exons. The open
reading frame (ORF) of the hBUBR1 gene comprises exons 1 through 23. There are
GC-rich regions located at the flanking region and about 150 bp upstream from
exon 1. The promoter region (424 bp upstream from exon 1) showed promoter
activity and includes multiple transcription factor consensus binding motifs,
including those for Sp1, Nkx-2, CdxA, SRY, MyoD, Ik-2, HNF-3b, Staf, Oct-1,
Nkx-2, v-Myb, and AML 1a. Multiple pathways leading to activation of those
binding factors may contribute to hBUBR1 gene transcription. Knowledge of the
genomic structure and the promoter region of the hBUBR1 gene will facilitate
investigation of its role in mitotic checkpoint control and tumor progression in
human cancers.

PMID: 12445743 [PubMed - indexed for MEDLINE]


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2: Gene. 2001 Oct 31;278(1-2):185-92. 

Genomic organization and transcription units of the human acyl-CoA synthetase 3
gene.

Minekura H, Kang MJ, Inagaki Y, Suzuki H, Sato H, Fujino T, Yamamoto TT.

Pharmacology and Molecular Biology Research Laboratories, Sankyo Co., Ltd.,
2-58, Hiromachi 1-Chome, Shinagawa-ku, Tokyo 140-8710, Japan.

Acyl-CoA synthetases (ACSs) play an essential role in fatty acid metabolism.
ACS3 is an arachidonate-preferring enzyme expressed in a wide range of human
tissues including brain, heart, placenta, prostate, skeletal muscle, testis and
thymus. As an initial step to understanding the transcriptional regulation of
the human ACS3 gene, we analyzed the genomic organization and transcription
units of the human ACS3 gene. Sequence analysis of genomic clones demonstrates
that the human ACS3 gene spans at least 80.6 kb and contains 17 exons. The human
ACS3 gene was mapped between the sequence-tagged site markers D2S360 and
WI-21901. Sequence inspection of the 5'-flanking region revealed potential DNA
elements including CCAAT, AP-1, Oct-1, GATAs, SRY, CdxA, Nkx-2.5, c-Myb, HSF2,
NF-AT, AP-2, NF-Y, and p300. A minimal promoter region required for the
expression of the human ACS3 gene in melanoma G361 cells was determined.

PMID: 11707336 [PubMed - indexed for MEDLINE]


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