1: Nucleic Acids Res. 1988 Aug 11;16(15):7527-44. 

Multiple nuclear factors interact with promoter sequences of the urokinase-type
plasminogen activator gene.

von der Ahe D, Pearson D, Nakagawa J, Rajput B, Nagamine Y.

Friedrich Miescher-Institut, Basel, Switzerland.

To characterize proteins that bind to the cyclic AMP inducible promoter of the
urokinase-type plasminogen activator gene, we performed a DNAase I footprinting
analysis. Within 500 nucleotides upstream of the transcription start site we
found eight protected regions due to at least four different binding proteins.
Among these is a single binding site for the transcription factor CTF/NF1, which
is flanked on each side by two conserved binding sites for the transcription
factor Sp1. A region at -380, which shares a similarity with sequences observed
in the corresponding regions of other cyclic AMP regulated genes, was protected.
This binding site contains a sequence of ten nucleotides which is repeated
further upstream at -480 and also protected against DNAase I digestion.
Comparisons of extracts from four different cell lines revealed that all DNA
binding factors are present in nuclei of uPA expressing and nonexpressing cells.
Mechanism underlying hormonal regulation of the gene is discussed.

PMID: 3412894 [PubMed - indexed for MEDLINE]


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