1: Leuk Res. 2005 Oct 7; [Epub ahead of print] 

Histologic and molecular characterizations of megakaryocytic leukemia in mice.

Hao X, Shin MS, Zhou JX, Lee CH, Qi CF, Naghashfar Z, Hartley JW, Fredrickson
TN, Ward JM, Morse HC 3rd.

Laboratory of Immunopathology, National Institute of Allergy and Infectious
Diseases, National Institute of Health, Rockville, MD 20852, USA.

Six cases of megakaryocytic leukemia (MKL) were identified and analyzed for
morphology and molecular features. MKL were composed of megakaryocyte lineage
cells ranging from immature to quite mature cells. VWF, GATA1 and RUNX1 were
strongly expressed in megakaryocytes in both normal spleen and MKL as analyzed
by immunohistochemistry (IHC). Altered expression of Meis1, Pbx1 and Psen2 and
Lef1 in MKL detected with oligonucleotide microarrays was confirmed by qPCR and
IHC. This is the first report of spontaneous MKL in mice, defining VWF as a
biomarker for diagnosis and suggesting possible involvement of a series of genes
in disease pathogenesis.

PMID: 16219351 [PubMed - as supplied by publisher]


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2: Gene. 2002 Jan 23;283(1-2):185-97. 

Functional and binding studies of HS3.2 of the beta-globin locus control region.

Molete JM, Petrykowska H, Sigg M, Miller W, Hardison R.

Department of Biochemistry and Molecular Biology, The Pennsylvania State
University, 206 Althouse Laboratory, University Park, PA 16802, USA

The distal locus control region (LCR) is required for high-level expression of
the complex of genes (HBBC) encoding the beta-like globins of mammals in
erythroid cells. Several major DNase hypersensitive sites (HSs 1-5) mark the
LCR. Sequence conservation and direct experimental evidence have implicated
sequences within and between the HS cores in function of the LCR. In this report
we confirm the mapping of a minor HS between HS3 and HS4, called HS3.2, and show
that sequences including it increase the number of random integration sites at
which a drug resistance gene is expressed. We also show that nuclear proteins
including GATA1 and Oct1 bind specifically to sequences within HS3.2. However,
the protein Pbx1, whose binding site is the best match to one highly conserved
sequence, does not bind strongly. GATA1 and Oct1 also bind in the HS cores of
the LCR and to promoters in HBBC. Their binding to this minor HS suggests that
they may be used in assembly of a large complex containing multiple regulatory
sequences.

PMID: 11867225 [PubMed - indexed for MEDLINE]


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