1: Cytogenet Cell Genet. 2001;94(1-2):49-54. 

Conserved synteny and gene order difference between human chromosome 12 and pig
chromosome 5.

Goureau A, Garrigues A, Tosser-Klopp G, Lahbib-Mansais Y, Chardon P, Yerle M.

Institut National de la Recherche Agronomique, Laboratoire de Genetique
Cellulaire, Castanet-Tolosan, France.

A comparative map of human chromosome 12 (HSA 12) and pig chromosome 5 (SSC 5)
was constructed using ten pig expressed sequence tags (ESTs). These ESTs were
isolated from primary granulosa cell cultures by differential display (EST
b10b), or from a granulosa cDNA library (VIIIE1, DRIM, N*9, RIIID2 and RVIC1) or
from a small intestine cDNA library (ATPSB, ITGB7, MYH9, and STAT2). Also used
were two Traced Orthologous Amplified Sequence Tags (TOASTs) (LALBA, TRA1), one
microsatellite-associated gene (IGF1) and finally five human YACs selected for
their cytogenetic position, with a view to increasing the number of informative
markers for the comparison. Large-insert clones were obtained by screening a pig
bacterial artificial chromosome (BAC) library with specific primers for each EST
and TOAST and for IGF1. These BACs were used as probes for fluorescent in situ
hybridisation (FISH) both on porcine and human metaphases. In addition, the
human YACs were FISH mapped on pig chromosomes. This allowed us to refine and,
in some cases, to correct the previous mapping obtained with a somatic cell
hybrid panel. While these data confirm chromosome painting results showing that
the distal part of SSC 5p arm is conserved on HSA 22, while the rest of the
chromosome corresponds to HSA 12, they also demonstrate gene-order differences
between human and pig. In addition, it was also possible to determine the
position of the synteny breakpoint. Copyright 2001 S. Karger AG, Basel

PMID: 11701954 [PubMed - indexed for MEDLINE]


---------------------------------------------------------------