1: Int J Oncol. 2005 May;26(5):1435-40. 

Comparative genomics on Vangl1 and Vangl2 genes.

Katoh Y, Katoh M.

M and M Medical BioInformatics, Hongo 113-0033, Japan.

WNT signals are transduced to the beta-catenin pathway or the planar cell
polarity (PCP) pathway. WNT - beta-catenin pathway is implicated in
carcinogenesis, while WNT-PCP pathway is implicated in cell motility and
metastasis. Drosophila Van Gogh (Vang), Frizzled (Fz), Starry night (Stan),
Prickle (Pk) and Diego (Dgo) are PCP signaling molecules. Vangl1 (Strabismus 2)
and Vangl2 (Strabismus 1 or Ltap) are mammalian homologs of Drosophila Vang
interacting with PRICKLE1, PRICKLE2, ANKRD6, DVL1, DVL2, DVL3, KAI1 and MAGI3.
Here we identified and characterized rat Vangl1 and Vangl2 genes by using
bioinformatics. Rat Vangl1 gene, consisting of eight exons, was located within
AC098913.7 and AC108524.6 genome sequences. Rat Vangl2 gene, consisting of eight
exons, was located within AC118856.3 and AC115243.5 genome sequences.
Exon-intron structure of mammalian Vangl1 and Vangl2 orthologs was well
conserved. E47 and double ELK1-binding sites were conserved among promoters of
mammalian Vangl1 orthologs. PAX4, NFkappaB, HNF4, SOX9, RFX1, and POU2F1
(OCT1)-binding sites were conserved among promoters of mammalian Vangl2
orthologs. Rat Vangl1 (526 aa) and Vangl2 (521 aa) were four-transmembrane
proteins with 71.5% total-amino-acid identity. Ser cluster motif
(SxxSxxSxxSxxSxxS) in the N-terminal cytoplasmic region and PDZ-binding motif in
the C-terminal cytoplasmic tail were evolutionarily conserved among vertebrate
Vangl1 and Vangl2 orthologs. This is the first report on rat Vangl1 and Vangl2
genes as well as on comparative genomics for Vangl1 and Vangl2 orthologs.

PMID: 15809738 [PubMed - indexed for MEDLINE]


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