1: Mamm Genome. 2000 Dec;11(12):1053-7. 

Genomic organization and mapping of mouse CDV (carnitine deficiency-associated
gene expressed in ventricle)-1 and its related CDV-1R gene.

Higashi M, Kobayashi K, Iijima M, Wakana S, Horiuchi M, Yasuda T, Yoshida G,
Kanmura Y, Saheki T.

Department of Biochemistry, Faculty of Medicine, Kagoshima University,
Sakuragaoka, Japan.

We have previously reported that CDV (carnitine deficiency-associated gene
expressed in ventricle)-1 was a downregulated gene in the hypertrophied
ventricle of carnitine-deficient juvenile visceral steatosis mice and that the
related gene (CDV-1R) showed no tissue specificity and no sensitivity to
carnitine deficiency. In the present paper, the CDV-1/1R gene was isolated from
a mouse genomic BAC library, and the genomic structure was characterized. We
found that the CDV-1/1R gene consisted of at least 19 exons and encompassed
approximately 48 kb. The splice sites conformed to the GT-AG rule, and the
CDV-1R mRNA containing 19 exons was processed. CDV-1 mRNA containing 5 exons was
constructed from the 3' half of CDV-1R. The first exon of CDV-1 consisted of the
3' side (116 bp) of intron 14 and exon 15 (87 bp) of CDV-1R. The presumed
promoter sequence for CDV-1 located in the intron 14 of CDV-1R contained the
common TATA box and consensus binding sites for various transcription factors
(Nkx-2.5, Spl, C/EBP, SRF, YY1, and CREB), which seem to play roles in the
heart-specific expression and carnitine deficiency-associated suppression of
CDV-1. In the upstream region of the CDV-1 promoter, we found two VNTRs, 13
repeats of GATA1, and 16 copies of STRE involved in yeast stress response. The
CDV-1/1R gene was located close to DSMIT68 on mouse Chromosome (Chr) 5,
corresponding to human Chr 12q24. All these data revealed that two mRNA species,
CDV-1 and CDV-1R, are expressed tissue-specifically by using promoters peculiar
to each transcript in a single gene.

PMID: 11130971 [PubMed - indexed for MEDLINE]


---------------------------------------------------------------