1: Genomics. 1997 Jul 15;43(2):156-64. 

Erratum in:
    Genomics 1997 Nov 1;45(3):633.

The human gene ZFP161 on 18p11.21-pter encodes a putative c-myc repressor and is
homologous to murine Zfp161 (Chr 17) and Zfp161-rs1 (X Chr)

Sobek-Klocke I, Disque-Kochem C, Ronsiek M, Klocke R, Jockusch H, Breuning A,
Ponstingl H, Rojas K, Overhauser J, Eichenlaub-Ritter U.

Faculty of Biology, Genetechnology/Microbiology Unit, University of Bielefeld,
Germany.

A clone from a lambda gt11 cDNA expression library of HeLa cells was isolated,
sequenced, and shown to encode a new human zinc finger protein. The cDNA of the
gene termed ZFP161 has an open reading frame of 1347 bp. The predicted protein
comprises 449 amino acid residues and contains five zinc finger motifs of the
Kruppel type near the C-terminus and a BTB/POZ domain in the N-terminal region.
The protein is 98% homologous to a murine zinc finger protein, ZF5 (M. Numoto et
al., 1993, Nucleic Acids Res. 21: 3767-3775), which is a putative
transcriptional repressor of c-myc and exhibits growth-suppressive activity in
mouse cell lines. Through the use of a panel of somatic cell hybrids for
chromosomal assignment and DNAs of somatic cell hybrids containing a deleted
chromosome 18 for fine mapping, the human gene ZFP161 was localized to
18p11.21-pter. Therefore, ZFP161 is a candidate gene by position for the
holoprosencephaly type 4 gene, HPE4, which is involved in congenital
malformations. With DNAs from an interspecific backcross, two homologous mouse
genes, Zfp161 and Zfp161-rs1, were mapped to chromosome 17 and the X chromosome,
respectively. Mapping of Zfp161 confirms and extends a region of homology
between distal mouse chromosome 17 and human 18p.

PMID: 9244432 [PubMed - indexed for MEDLINE]


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